Food Safety and Microbiology
Unit presentation
SECALIM is a Mixt Research Unit (MRU 1014) INRA/Oniris, with 21 permanent people, specialised in microbiological quality of food. Its research is now focused on the contaminated food considered as a specific ecosystem. This contaminated food (food-ecosystem) can be considered as an « organ » potentially subjected to disruption or influenced by its environment. This involves all the elements and factors that can impact survival, growth, regulation (or disregulation), alteration capacity and virulence of microorganisms. The overall goal of SECALIM is to answer the following question : “How food environments encountered throughout the food processing chain can influence the microbial risks, especially Campylobacter risk ?”
Unit members
Director
Hervé PREVOST - Professor
Contact : herve.prevost(at)oniris-nantes.fr
Co-Director
Odile TRESSE - Researcher
contact : odile.tresse(at)oniris-nantes.fr
Researchers and Professors
Catherine MAGRAS – Professor, HDR
Marie-France PILET – Associate professor, HDR
Jean-Michel CAPPELIER – Associate professor, HDR
Xavier DOUSSET – Professor, HDR
Michel FEDERIGHI – Professor, HDR
Nabila HADDAD – Associate professor
Hervé PREVOST – Professor, HDR
Odile TRESSE – Researcher INRA, HDR
Jeanne-Marie MEMBRE – Research engineer INRA, HDR
Sandrine GUILLOU – Research engineer
Scientific Publications
Publications in 2011
Belguesmia Y, Madi A, Sperandio D, Merieau A, Feuilloley M, Prevost H, Drider D, Connil N (2011) Growing insights into the safety of bacteriocins: the case of enterocin S37. Research in Microbiology 162, 159-163.
Chihib NE, Mary P, Federighi M, Hornez JP (2011) The effect of preculture conditions on the culturability and viability of Aeromonas hydrophila when exposed to oligotrophic and cold stresses. World Journal of Microbiology & Biotechnology 27, 417-422.
Fosse J, Laroche M, Oudot N, Seegers H, Magras C (2011) On-farm multi-contamination of pigs by food-borne bacterial zoonotic hazards: An exploratory study. Veterinary Microbiology 147, 209-213.
Jaffres E, Lalanne V, Mace S (2011) Sensory characteristics of spoilage and volatile compounds associated with bacteria isolated form cooked and peeled tropical shrimps using SPME-GC-MS analyses. International Journal of Food Microbiology 147, 195-202.
Lerasle M, Guillou S, Simonin H, Laroche M, de Lamballerie M, Federighi M (2011) Membrane integrity of Campylobacter jejuni subjected to high pressure is pH-dependent. High Pressure Research, 1-8.
Membre JM, Laroche M, Magras C (2011) Assessment of levels of bacterial contamination of large wild game meat in Europe. Food Microbiology 28, 1072-1079.
Membre JM, Van Zuijlen A (2011) A probabilistic approach to determine thermal process setting parameters: application for commercial sterility of products. International Journal of Food Microbiology 144, 413-420.
Messaoudi S, Kergourlay G, Rossero A, Ferchici M, Prevost H, Drider D, Manai M, Dousset X (2011) Identification of lactobacilli residing in chicken ceca with antagonism against Campylobacter. International Microbiology 14, 103-110.
Pilet MF, Leroi F (2011) Applications of protective cultures, bacteriocins and bacteriophages in fresh seafood and seafood products. In 'Protective Cultures, Antimicrobial Metabolites and Bacteriophages for Food and Beverage Biopreservation' pp. 324-347.
Rihakova J, Prevost H, Demnerova K, Drider D (2011) Effect of recombinant divercin RV41, structural variants and the activators of potassium channels on Listeria monocytogenes EGDe. Folia Microbiologica 56, 110-115.
Todorov SD, Rachman C, Fourrier A, Dicks LMT, van Reenen CA, Prevost N, Dousset X (2011) Characterization of a bacteriocin produced by Lactobacillus sakei R1333 isolated from smoked salmon. Anaerobe 17, 23-31.
Publications in 2010
Ben Said, N.E., Federighi, M., Bakhrouf, A., Orange, N., 2010, Effectiveness of Pulsed Ultraviolet-Light Treatment for Bacterial Inactivation on Agar Surface and Liquid Medium. Foodborne Pathogens and Disease 7, 1401-1406.
Bieche, C., de Lamballerie, M., Federighi, M., Le Bail, A., Tresse, O., 2010, Proteins involved in Campylobacter jejuni 81-176 recovery after high-pressure treatment, In: Bartlett, D.H. (Ed.) High-Pressure Bioscience and Biotechnology. Blackwell Publishing, Oxford, pp. 133-138.
Duodu, S., Holst-Jensen, A., Skjerdal, T., Cappelier, J.M., Pilet, M.F., Loncarevic, S., 2010, Influence of storage temperature on gene expression and virulence potential of Listeria monocytogenes strains grown in a salmon matrix. Food Microbiology 27, 795-801.
Fall, P.A., Leroi, F., Cardinal, M., Chevalier, F., Pilet, M.F., 2010, Inhibition of Brochothrix thermosphacta and sensory improvement of tropical peeled cooked shrimp by Lactococcus piscium CNCM I-4031. Letters in Applied Microbiology 50, 357-361.
Fall, P.A., Leroi, F., Chevalier, F., Guerin, C., Pilet, M.F., 2010, Protective Effect of a Non-Bacteriocinogenic Lactococcus piscium CNCM I-4031 Strain Against Listeria monocytogenes in Sterilized Tropical Cooked Peeled Shrimp. Journal of Aquatic Food Product Technology 19, 84-92.
Fosse, J., Laroche, M., Oudot, N., Rossero, A., Seegers, H., Magras, C., 2010, Farm to slaughter study of pigs batches infection by Staphylococcus aureus and Yersinia enterocolitica: an exploratory study. Rev. Med. Vet. 161, 20-29.
Garnier, M., Matamoros, S., Chevret, D., Pilet, M.F., Leroi, F., Tresse, O., 2010, Adaptation to Cold and Proteomic Responses of the Psychrotrophic Biopreservative Lactococcus piscium Strain CNCM I-4031. Appl. Environ. Microbiol. 76, 8011-8018.
Haddad, N., Maillart, G., Garenaux, A., Jugiau, F., Federighi, M., Cappelier, J.M., 2010, Adhesion Ability of Campylobacter jejuni to Ht-29 Cells Increases with the Augmentation of Oxidant Agent Concentration. Curr. Microbiol. 61, 500-505.
Haddad, N., Marce, C., Magras, C., Cappelier, J.M., 2010, An Overview of Methods Used To Clarify Pathogenesis Mechanisms of Campylobacter jejuni. Journal of Food Protection 73, 786-802.
Ignatova, M., Prevost, H., Leguerinel, I., Guillou, S., 2010, Growth and reducing capacity of Listeria monocytogenes under different initial redox potential. Journal of Applied Microbiology 108, 256-265.
Jaffres, E., Prevost, H., Rossero, A., Joffraud, J.J., Dousset, X., 2010, Vagococcus penaei sp. nov., isolated from spoilage microbiota of cooked shrimp (Penaeus vannamei). International Journal of Systematic and Evolutionary Microbiology 60, 2159-2164.
Kerouanton, A., Roche, S.M., Marault, M., Velge, P., Pourcher, A.M., Brisabois, A., Federighi, M., Garrec, N., 2010, Characterization of isolates of Listeria monocytogenes from sludge using pulsed-field gel electrophoresis and virulence assays. Journal of Applied Microbiology 108, 1380-1388.
Matamoros, S., Andre, S., Hue, I., Prevost, H., Pilet, M.F., 2010, Identification of lactic acid bacteria involved in the spoilage of pasteurized "foie gras" products. Meat Science 85, 467-471.
Rihakova, J., Cappelier, J.M., Hue, I., Demnerova, K., Federighi, M., Prevost, H., Drider, D., 2010, In Vivo Activities of Recombinant Divercin V41 and Its Structural Variants against Listeria monocytogenes. Antimicrobial Agents and Chemotherapy 54, 563-564.
Rivoal, K., Queguiner, S., Boscher, E., Bougeard, S., Ermel, G., Salvat, G., Federighi, M., Jugiau, F., Protais, J., 2010, Detection of Listeria monocytogenes in raw and pasteurized liquid whole eggs and characterization by PFGE. International Journal of Food Microbiology 138, 56-62.
Sulaeman, S., Le Bihan, G., Rossero, A., Federighi, M., De, E., Tresse, O., 2010, Comparison between the biofilm initiation of Campylobacter jejuni and Campylobacter coli strains to an inert surface using BioFilm Ring Test®. Journal of Applied Microbiology 108, 1303-1312.
Todorov, S.D., Wachsman, M., Tome, E., Dousset, X., Destro, M.T., Dicks, L.M.T., Franco, B., Vaz-Velho, M., Drider, D., 2010, Characterisation of an antiviral pediocin-like bacteriocin produced by Enterococcus faecium. Food Microbiology 27, 869-879.
Doctoral thesis (2006-2011)
Ségolène CALVEZ : Mécanisme moléculaire de résistance aux bactériocines de la classe lia. Soutenue en 2007.
Noura ELMNASSER : Effet de stress technologiques sur la survie de différents modèles bactériens : applications particulières aux stress thermique, osmotique, acide et la lumière pulsée. Soutenue en 2007.
Amélie GARENAUX : Campylobacter jejuni et stress oxydant : étude de la survie à différentes températures et recherche des mécanismes de défense par approches protéomique et transcriptomie. Soutenue en 2007.
Morgan GUILBAUD : Caractérisation et maîtrise des écosystèmes microbiens dans les industries de transformation des produits de la mer. Soutenue en 2007.
Karim NAGHMOUCHI : Contribution à l’étude du mécanisme d’action de la divergicine M35, une nouvelle bactériocine produite par Carnobacterium divergens M35. Soutenue en 2007.
Sébastien MATARAMOS : Caractérisation de bactéries lactiques psychotropes en vue de leur utilisation dans la biopréservation des poissons. Soutenue en 2008.
Milly LEBLANC MARIDOR : Campylobacter chez le porc : méthodes d’identification quantitative et dynamique d’infection. Soutenue en 2008.
Julien FOSSE : Valeur informative d’indicateurs ante et post-mortem pour la détection des dangers biologiques pour le consommateur de viande porcine. Soutenue en 2008.
Marya IGNATOVA : Effets combinés du pH de la température et du potentiel d’oxydo-réduction sur le comportement de Listeria monocytogenes. Soutenue en 2009.
Emmanuel JAFFRES : Caractérisation des écosystèmes microbiens complexes en vue de la maîtrise des flores pathogènes et d’altération des produits de la mer. Soutenue en 2009.
Jitka RIHAKOVA : Ingénierie des peptides antimicrobiens et applications médicales. Soutenue en 2009.
Yanath BELGUESMIA : Purification et caractérisation du mode d’action et de l’innocuité de l’entérocine S37, une bactériocine produite par Enterococcus faecalis S37. Soutenue en 2010.
Nabila HADDAD : Aspects moléculaires et cellulaires de la biologie. Soutenue en 2010.
Clémence BIECHE : Effet des hautes pressions sur Campylobacter jejuni : étude des mécanismes d’inactivation. Soutenue en 2010.
Papa Abdoulaye FALL : Impacts et modélisation des paramètres physico-chimiques et des interactions bactériennes sur la croissance de microorganismes pathogènes et altérants : application à la crevette tropicale. Soutenue en 2011.
Sheiam Sulaeman : Rôle des protéines membranaires dans l'adaptation de Campylobacter jejuni à son environnement. Soutenue en 2011.
Soumaya MESSAOUDI : Lactobacillus salivarius SMXD 51 isolé du caecum de poulet : caractérisation de sa batériosine anti-campylobacter et de son potentiel probiotique. Soutenue en 2012.
